HOT FIREPol® Blend Master Mix Ready to Load

Description

Ready to load hot-start PCR master mix for more demanding PCR assaysincreased yield, sensitivity and specificityup to 5x higher fidelitysuitable for templates up to 5 kbreduced primer dimer formationload directly on gel after PCRMgCl2 (mM) final concentration 2.05 x 1ml | 1250 rxnDescriptionHOT FIREPol Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water), an additional compound needed for direct loading onto an agarose gel, and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.HOT FIREPol Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase.In addition, the mix also allows the amplification of longer fragments.Generated PCR products are compatible with blunt-end and T/A cloning procedures.RecommendationsWe recommend using HOT FIREPol Blend Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.PropertiesConcentration: 5xHot-start: yes, initial activation in 12-15 minReady to load: yesFidelity: 5 x TaqCloning Type: T/A cloning and Blunt-end cloningAmplicon Size: up to 5 kbMix ComponentsHOT FIREPol DNA polymerase: chemically modified FIREPol DNA Polymerase enabeling hot-startProofreading enzyme: to enhance fidelity5x Blend Master Mix Bufferwith 7. 5